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产品描述：人膜联蛋白Ⅱ(ANX-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、唾液、细胞裂解物样本中的ANXA2含量。ANXA2 即膜联蛋白 A2，是一种钙离子依赖的磷脂结合蛋白。它参与细胞内吞、外吐等膜转运过程。在研究机制方面，其可在多种癌症中高表达，与肿瘤的增殖、迁移、侵袭及血管生成等密切相关，是潜在的癌症诊断标志物与治疗靶点。试剂盒检测范围为0.39 ng/mL-25 ng/mL，适用于细胞生物学研究、疾病机制探索以及生物标志物筛选等科研场景，通过定量分析 ANX - Ⅱ在体液或细胞内的动态变化，为研究其在炎症反应、血栓形成或肿瘤微环境中的作用提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Annexin A2 ELISA Kit; Annexin II ELISA Kit; Annexin II; heavy chain ELISA Kit; Annexin-2 ELISA Kit; ANX 2 ELISA Kit; ANX2 ELISA Kit; ANX2L4 ELISA Kit; ANXA2 ELISA Kit; ANXA2_HUMAN ELISA Kit; arylsulfatase B ELISA Kit; CAL1H ELISA Kit; Calpactin I heavy chain ELISA Kit; calpactin I heavy polypeptide (p36) ELISA Kit; Calpactin I heavy polypeptide ELISA Kit; Calpactin-1 heavy chain ELISA Kit; chromobindin 8 ELISA Kit; Chromobindin-8 ELISA Kit; Epididymis secretory protein Li 270 ELISA Kit; HEL S 270 ELISA Kit; LIP2 ELISA Kit; Lipocortin II ELISA Kit; LPC2 ELISA Kit; LPC2D ELISA Kit; p36 ELISA Kit; P36 protein ELISA Kit; PAP-IV ELISA Kit; Placental anticoagulant protein IV ELISA Kit; Protein I ELISA Kit
缩写：ANXA2
Uniprot No.：P07355
种属：Homo sapiens (Human)
样本类型：serum, urine, saliva, cell lysates
检测范围：0.39 ng/mL-25 ng/mL
灵敏度：0.098 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 96-110 | |||||
回收率：
| The recovery of human ANX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 252.7482.8122.7802.659 12.52.1312.1762.1542.033 6.251.4911.4041.4481.327 3.120.8660.8390.8530.732 1.560.5460.5240.5350.414 0.780.3630.3910.3770.256 0.390.2540.2690.2620.141 00.1250.1170.121 | ||||||
货期：3-5 working days
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