首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人膜内氨酰氨基肽酶(ANPEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANPEP含量。ANPEP即氨肽酶N，是一种膜结合糖蛋白。它在多种生理和病理过程如血管生成、细胞迁移等中发挥作用。研究机制上，其可促进肿瘤细胞生长、侵袭和转移，还与病毒感染相关，是药物研发的重要靶点，针对它的抑制剂研究正持续开展。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于科研领域中对ANPEP表达调控机制的研究，如肿瘤微环境分析、免疫细胞功能探索或代谢相关疾病模型构建等应用场景，尤其适用于需要同时检测体液样本（血清/血浆）和组织样本的研究设计。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alanyl (membrane) aminopeptidase ELISA Kit; Alanyl aminopeptidase ELISA Kit; Aminopeptidase M ELISA Kit; Aminopeptidase N ELISA Kit; AMPN_HUMAN ELISA Kit; ANPEP ELISA Kit; AP M ELISA Kit; AP N ELISA Kit; AP-M ELISA Kit; AP-N ELISA Kit; APN ELISA Kit; CD 13 ELISA Kit; CD13 ELISA Kit; CD13 antigen ELISA Kit; gp150 ELISA Kit; hAPN ELISA Kit; LAP 1 ELISA Kit; LAP1 ELISA Kit; Microsomal aminopeptidase ELISA Kit; Myeloid plasma membrane glycoprotein CD13 ELISA Kit; p150 ELISA Kit; PEPN ELISA Kit
缩写：ANPEP
Uniprot No.：P15144
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 84-99 | |||||
| 1:40 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:160 | Average % | 103 | ||||
| Range % | 96-109 | |||||
回收率：
| The recovery of human ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6272.6452.6362.54510002.1312.2982.2152.1245001.4581.5021.4801.3892500.7310.7550.7430.6521250.3770.3520.3650.27462.50.2010.2120.2070.11631.250.1890.1880.1890.09800.0870.0940.091 | ||||||
货期：3-5 working days
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