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产品描述:人锚蛋白重复结构域蛋白26(ANKRD26)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ANKRD26含量。ANKRD26是一个受关注的靶点。其它在人体生理过程可能扮演重要角色。研究机制上,具体作用机制仍在探索,可能参与细胞内一些信号传导通路等,深入研究它有望为相关疾病的发病机制、诊断及治疗提供新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于细胞分化、造血调控机制等基础研究,或疾病模型中ANKRD26表达动态变化的定量分析;为肿瘤生物学、血液病理学等科研领域提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Ankrd26 ELISA Kit; Ankyrin repeat domain 26 ELISA Kit; Ankyrin repeat domain-containing protein 26 ELISA Kit; Ankyrin repeat domaincontaining protein 26 ELISA Kit; ANR26_HUMAN ELISA Kit; bA145E8.1 ELISA Kit; KIAA1074 ELISA Kit
缩写:ANKRD26
Uniprot No.:Q9UPS8
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.81 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANKRD26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 90 | |||||
Range % | 87-93 | ||||||
1:2 | Average % | 102 | |||||
Range % | 98-106 | ||||||
1:4 | Average % | 85 | |||||
Range % | 80-91 | ||||||
1:8 | Average % | 104 | |||||
Range % | 101-108 |
回收率:
The recovery of human ANKRD26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 99 | 95-103 | |||||
EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected20002.1262.2012.1642.03910001.6711.6581.6651.5405001.1351.2041.1701.0452500.6960.6850.6910.5661250.4640.4710.4680.34362.50.3180.3200.3190.19431.250.2410.2490.2450.12000.1260.1240.125 |
货期:3-5 working days
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