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产品描述：大鼠血管生成素相关蛋白4(ANGPTL4)酶联免疫试剂盒大鼠血管生成素相关蛋白4(ANGPTL4)酶联免疫试剂盒检测血清、血浆、组织培养上清液、组织匀浆、支气管肺泡灌洗液、尿液样本中的ANGPTL4含量。ANGPTL4是一个重要靶点。它属于血管生成素样蛋白家族，在脂质代谢、血管生成等生理过程中发挥作用。研究发现，它可抑制脂蛋白脂肪酶活性影响甘油三酯代谢。同时其在肿瘤血管生成等病理机制中也有参与，为相关疾病治疗提供了潜在方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品适用于肥胖、糖尿病、动脉粥样硬化等代谢相关疾病的机制研究，或炎症模型、血管新生实验的分子水平分析，为体外实验提供高灵敏度的定量检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angptl4Angiopoietin-related protein 4 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
缩写：ANGPTL4
Uniprot No.：Q6TMA8
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 87 | |
| Range % | 83-102 | ||
| 1:400 | Average % | 86 | |
| Range % | 81-90 | ||
| 1:800 | Average % | 92 | |
| Range % | 87-98 | ||
| 1:1600 | Average % | 102 | |
| Range % | 99-105 |
回收率：
| The recovery of rat ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.6342.6872.6612.561 2502.2262.2682.2472.147 1251.5681.5341.5511.451 62.50.9680.9430.9560.856 31.250.5270.5680.5480.448 15.620.3980.3770.3880.288 7.80.2740.2890.2820.182 00.0990.1010.100 | ||||||
货期：3-5 working days
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