首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管生成素相关蛋白2(ANGPTL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ANGPTL2含量。ANGPTL2是一种与血管生成素结构相似的蛋白，参与血管生成、脂质代谢和炎症调节。其通过自分泌和旁分泌机制诱导内皮细胞出芽，在血管生成中发挥关键作用。研究显示，ANGPTL2通过PirB激活Müller细胞，促进炎症因子释放，参与眼内炎症。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒通过高特异性抗体对靶蛋白进行捕获与检测，操作便捷且重复性良好，可为体外研究ANGPTL2在肥胖、糖尿病、动脉粥样硬化等疾病模型中的功能机制提供可靠工具，亦可用于评估药物干预或基因编辑后生物样本中ANGPTL2的动态变化，满足基础科研与药物开发中的蛋白定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angptl2 ELISA Kit; Arp2Angiopoietin-related protein 2 ELISA Kit; Angiopoietin-like protein 2 ELISA Kit
缩写：ANGPTL2
Uniprot No.：Q9R045
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 92-96 | |||||
回收率：
| The recovery of mouse ANGPTL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 101-106 | ||||
| EDTA plasma (n=4) | 95 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2212.1572.1892.0325001.5101.4661.4881.3312500.8960.8880.8920.7351250.5020.4940.4980.34162.50.3320.3440.3380.18131.20.2840.2950.2900.13315.60.2110.2290.2200.06300.1510.1630.157 | ||||||
货期：3-5 working days
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