首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管生成素样蛋白4 (ANGPTL4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPTL4含量。ANGPTL4是一种重要靶点。它是血管生成素样蛋白家族成员，在脂质代谢、血管生成等生理过程发挥作用。研究其机制主要聚焦于它对脂蛋白脂肪酶活性的调节，以及在肿瘤血管生成和转移中的影响，有望为相关疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于基础科研中探索ANGPTL4在代谢调控、心血管病理机制及肿瘤相关信号通路中的作用，可为细胞实验、动物模型等研究提供精准的蛋白表达数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angptl4 ELISA kit; Farp ELISA kit; Fiaf ELISA kit; Ng27Angiopoietin-related protein 4 ELISA kit; 425O18-1 ELISA kit; Angiopoietin-like protein 4 ELISA kit; Fasting-induced adipose factor ELISA kit; Hepatic fibrinogen/angiopoietin-related protein ELISA kit; HFARP ELISA kit; Secreted protein Bk89) [Cleaved into: ANGPTL4 N-terminal chain; ANGPTL4 C-terminal chain] ELISA kit
缩写：ANGPTL4
Uniprot No.：Q9Z1P8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of mouse ANGPTL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 99-104 | ||||
| EDTA plasma (n=4) | 88 | 85-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1000.1020.101 1000.1460.1530.150 500.2540.2470.251 250.3570.3680.363 12.50.4660.4570.462 6.250.6010.6380.620 3.1250.8420.8720.857 01.6211.5981.610 | ||||||
货期：3-5 working days
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