首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管生成素样蛋白2(ANGPTL2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ANGPTL2含量。ANGPTL2是一个有研究价值的靶点。它在多种生理和病理过程中扮演角色，如参与炎症反应、血管生成等。研究机制方面，主要通过影响细胞增殖、迁移等活动参与疾病发生发展，对其深入研究有望为相关疾病治疗提供新思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外实验研究，可用于探索疾病模型中ANGPTL2的表达特征，如分析肥胖、动脉粥样硬化或癌症微环境样本的蛋白水平变化，或评估药物干预、基因编辑等处理对ANGPTL2分泌的影响，为代谢调控机制、病理生理学及靶向治疗研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI593246 ELISA Kit; Angiopoietin like 2 ELISA Kit; Angiopoietin related protein 2 ELISA Kit; Angiopoietin-like protein 2 ELISA Kit; Angiopoietin-related protein 2 ELISA Kit; ANGL2_HUMAN ELISA Kit; Angptl2 ELISA Kit; Arp2 ELISA Kit; AW260363 ELISA Kit; HARP ELISA Kit; MGC8889 ELISA Kit; UNQ170/PRO196 ELISA Kit
缩写：ANGPTL2
Uniprot No.：Q9UKU9
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ANGPTL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human ANGPTL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.6212.6842.6532.479502.2182.3362.2772.103251.7031.7291.7161.54212.51.0121.0651.0390.8656.250.5750.5980.5870.4133.120.3570.3690.3630.1891.560.2460.2580.2520.07800.1730.1750.174 | ||||||
货期：3-5 working days
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