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产品描述：猪血管生成素2(ANG-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPT2含量。ANGPT2是一个备受关注的靶点。它在血管生成、血管稳态维持等方面发挥重要作用。其研究机制主要围绕调控内皮细胞功能、影响血管通透性等。针对它的研究有望为肿瘤、心血管疾病等提供新的治疗策略和药物研发方向。试剂盒检测范围为0.94 ng/mL-60 ng/mL，适用于科研场景下猪模型的组织病理分析、血清/血浆循环标志物动态监测，或药物干预后血管生成相关通路的机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
缩写：ANGPT2
Uniprot No.：Q9BDY7
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.94 ng/mL-60 ng/mL
灵敏度：0.235 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.5092.5962.5532.401301.7491.7681.7591.607151.0041.0681.0360.8847.50.6630.6420.6530.5013.750.3900.3780.3840.2321.880.2980.2900.2940.1420.940.2240.2390.2320.08000.1510.1530.152 | ||||||
货期：3-5 working days
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