首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管生成素2(ANG-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ANGPT2含量。ANGPT2是一个重要靶点。它在血管生成、血管稳定性维持等生理过程中发挥作用。研究发现其异常表达与肿瘤血管生成、肿瘤生长及转移等密切相关。相关机制研究聚焦于抑制其活性或阻断其信号通路，以实现抗肿瘤等潜在治疗目的。试剂盒检测范围为0.156 pg/mL-10 pg/mL，适用于体外实验环境下的小鼠疾病模型研究，如肿瘤微环境分析、血管异常增生机制探索或炎症相关信号通路研究，为心血管疾病、癌症生物学等领域的科研工作提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angpt2 ELISA Kit; Agpt2Angiopoietin-2 ELISA Kit; ANG-2 ELISA Kit
缩写：ANGPT2
Uniprot No.：O35608
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 pg/mL-10 pg/mL
灵敏度：0.039 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:40 | Average % | 105 | ||||
| Range % | 100-107 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of mouse ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected102.4272.4112.4192.25051.9882.0222.0051.8362.51.3541.3791.3671.1981.250.7800.7420.7610.5920.6250.4950.4640.4800.3110.3120.3620.3770.3700.2010.1560.2650.2550.2600.09100.1710.1670.169 | ||||||
货期：3-5 working days
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