首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管生成素1(ANG-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPT1含量。ANGPT1即血管生成素1，为血管生成素家族成员，在血管生成、维持血管稳定和调节内皮细胞功能等方面起重要作用。其研究机制主要围绕与受体Tie-2结合，激活下游信号通路，促进内皮细胞存活、迁移和血管成熟。相关研究有助于探索血管相关疾病治疗策略。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于研究血管生成相关疾病模型（如缺血再灌注损伤、肿瘤血管化）中ANG - 1的动态变化，或探索其与血管内皮生长因子（VEGF）等分子的协同调控机制，为心血管生物学、肿瘤微环境及再生医学等科研领域提供灵敏、特异的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Angpt1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
缩写：ANGPT1
Uniprot No.：O35460
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 93-109 | ||||||
| 1:2 | Average % | 112 | |||||
| Range % | 103-116 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 84-98 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
回收率：
| The recovery of rat ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 83-94 | |||||
| EDTA plasma (n=4) | 103 | 92-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected4002.9852.8452.9152.7812002.6812.5792.6302.4961002.2972.2132.2552.121501.6841.6221.6531.519251.0010.9560.9790.84512.50.4490.4410.4450.3116.250.2510.2420.2470.11300.1320.1360.134 | |||||||
货期：3-5 working days
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