Pig Angiopoietin 1,ANG-1 ELISA Kit

货号: IPD-E08457p

Price： ￥3000

48T 96T

数量：

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产品描述：猪血管生成素1(ANG-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPT1含量。ANGPT1是一个热门靶点。它是血管生成素家族成员，在血管形成、稳定及内皮细胞功能维持中有重要作用。研究机制主要围绕其与Tie2受体相互作用，调控血管的发育、重塑等过程，为心血管疾病等治疗提供潜在方向。试剂盒检测范围为6 ng/mL-200 ng/mL，心血管疾病机制研究、血管生成相关动物模型分析以及炎症或组织修复过程中的分子调控探索等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANGPT1Angiopoietin-1 ELISA kit; ANG-1 ELISA kit
缩写：ANGPT1
Uniprot No.：Q9BDY8
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：6 ng/mL-200 ng/mL
灵敏度：6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	95
	Range %	85-100
1:4	Average %	89
	Range %	85-101
1:8	Average %	90
	Range %	80-99

回收率：

The recovery of pig ANG-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2Average 2000.3450.3510.348 1000.4780.4860.482 500.7030.7160.710 171.0681.1031.086 61.3161.3551.336 01.9251.9681.947

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