首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Ang1-7含量。Ang1-7是有研究价值的靶点。它在心血管、肾脏等多个系统发挥作用，具有舒张血管、抗增殖、抗炎等效应。研究机制方面，主要通过与Mas受体结合，激活下游信号通路，调节细胞功能，改善器官病理生理状态。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于实验室对小鼠疾病模型中Ang1-7的动态监测，如高血压、心肌肥厚、肾脏损伤等研究的生物标志物分析，或体外药物筛选实验中细胞因子分泌水平的定量评估。Ang1-7水平变化常被用于心血管疾病模型研究、代谢综合征机制探索及药物干预效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Ang1-7
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.7452.7212.7332.5972002.3162.2452.2812.1451001.9231.8981.9111.775501.4671.3661.4171.281250.7960.7880.7920.65612.50.4580.4470.4530.3176.250.2580.2420.2500.11400.1380.1340.136 | ||||||
货期：3-5 working days
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