| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SSMED |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human XRCC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于XRCC4抗体的3篇参考文献及其摘要概述：
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1. **"XRCC4 protein interacts with DNA ligase IV and is required for double-strand break repair and V(D)J recombination"**
- **作者**: Critchlow, S.E. et al.
- **摘要**: 该研究通过免疫共沉淀实验(使用XRCC4抗体)揭示了XRCC4与DNA连接酶IV(Ligase IV)的相互作用，证明两者是DNA双链断裂修复及V(D)J重组的必需复合物。研究还发现，XRCC4缺陷的细胞对电离辐射高度敏感。
2. **"Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding, and inability to support V(D)J recombination"**
- **作者**: Li, Z. et al.
- **摘要**: 文章利用XRCC4抗体进行Western blot分析，发现Ku70缺陷的小鼠胚胎干细胞中，XRCC4与DNA-PK复合物的结合能力受损，导致DNA末端连接功能缺陷，进一步验证了XRCC4在NHEJ修复途径中的核心作用。
3. **"Phosphorylation of XRCC4 regulates its interaction with the DNA repair protein PAXX and modulates DNA damage response"**
- **作者**: Liu, Y. et al.
- **摘要**: 通过特异性XRCC4抗体检测，研究发现XRCC4的磷酸化修饰(如Ser260位点)可调控其与PAXX蛋白的结合，影响DNA损伤修复效率。该发现为理解XRCC4在基因组稳定性维持中的动态调控提供了新机制。
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以上文献均涉及XRCC4抗体的应用(如免疫沉淀、Western blot等)，并聚焦于其在DNA修复、蛋白互作及疾病模型中的功能。如需更早期或特定方向的研究，可进一步补充。
**Background of XRCC4 Antibody**
XRCC4 (X-ray repair cross-complementing protein 4) is a critical DNA repair protein involved in the non-homologous end joining (NHEJ) pathway, the primary mechanism for repairing DNA double-strand breaks (DSBs) in mammals. It functions as a stabilizing scaffold, interacting with DNA ligase IV to facilitate the final ligation step of broken DNA ends. XRCC4 also associates with other NHEJ components, such as XLF (XRCC4-like factor) and the Ku70/Ku80 heterodimer, ensuring genomic stability and preventing chromosomal aberrations.
Antibodies targeting XRCC4 are essential tools for studying its expression, localization, and interactions in DNA repair processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate XRCC4’s role in maintaining genomic integrity, particularly in contexts of radiation damage, chemotherapy response, or genetic disorders linked to NHEJ dysfunction. Dysregulation or mutations in XRCC4 are associated with immunodeficiency, neurological defects, and cancer susceptibility, highlighting its clinical relevance.
XRCC4 antibodies are often validated for specificity across human, mouse, and rat models, aiding translational research. Their applications extend to exploring therapeutic targets for cancers with defective DNA repair mechanisms and understanding resistance to DNA-damaging treatments.
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