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产品描述：人血管紧张素1-7（Ang1-7）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的Ang1-7含量。Ang1-7是一种血管紧张素，具有血管舒张作用，影响心血管器官如心脏、血管和肾脏。研究显示，Ang1-7通过降低ERK1/2MAPK活性，调节心血管功能，并在肺纤维化、肝癌转移等疾病中发挥作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于心血管疾病机制研究、药物干预效果评估、细胞模型中Ang1-7分泌水平检测等科研领域，尤其适合低丰度样本的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Ang1-7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-109 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 92-114 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-110 | |||||
回收率：
| The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-110 | ||||
| EDTA plasma (n=4) | 99 | 90-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.6672.6842.6762.5252501.9341.9391.9371.7861251.1231.1191.1210.97062.50.6900.6740.6820.53131.20.4620.4710.4670.31615.60.3160.3060.3110.1607.80.2360.2250.2310.08000.1520.1500.151 | ||||||
货期：3-5 working days
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