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产品描述：猪血管生长素(ANG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANG含量。猪血管生长素（ANG）是RNA酶A超家族成员，具有刺激血管生长的功能。研究发现，ANG基因可能与宿主防御有关，其变异较大，对系统先天免疫很重要。ANG蛋白对多种病原微生物具有杀伤力，在抗病育种和疾病治疗中具有潜在应用价值。试剂盒检测范围为94 pg/mL-6000 pg/mL，适用于心血管疾病模型研究、再生医学实验、炎症相关血管病理分析等科研场景，特别在猪源样本的血管生成机制探索中具有重要应用价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANGAngiogenin ELISA kit; EC 3.1.27.- ELISA kit; Ribonuclease 5 ELISA kit; RNase 5 ELISA kit
缩写：ANG
Uniprot No.：P31346
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：94 pg/mL-6000 pg/mL
灵敏度：47 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 60000.1290.1280.129 30000.2610.2560.259 15000.5090.5290.519 7500.9320.9410.937 3751.4011.3941.398 1881.7531.7421.748 942.0872.0072.047 02.4762.5542.515 | ||||||
货期：3-5 working days
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