首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠α1微球蛋白/bikunin蛋白前体(AMBP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AMBP含量。AMBP是一个靶点。它是血管紧张素原结合蛋白，在血压调节等生理过程中发挥作用。研究机制方面，可能涉及与血管紧张素等的相互作用来影响心血管系统。但目前相关研究或许还在不断深入探索其在疾病发生发展中的具体角色和潜在应用。试剂盒检测范围为1.563 ng/mL-100 ng/mL，本试剂盒适用于基础科研中探索AMBP在急慢性炎症、组织损伤修复或代谢性疾病模型中的动态变化，亦可用于评估药物干预或基因修饰对AMBP表达的影响。助力于肾脏生理学、肝脏病理学及炎症调控机制等领域的研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
缩写：AMBP
Uniprot No.：Q07456
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：1.563 ng/mL-100 ng/mL
灵敏度：0.649 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:200 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:400 | Average % | 94 | |||||
| Range % | 90-97 | ||||||
| 1:800 | Average % | 91 | |||||
| Range % | 84-95 | ||||||
回收率：
| The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-93 | |||||
| EDTA plasma (n=4) | 97 | 92-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 1000.1400.1450.143 500.3570.3660.362 250.5690.5780.574 12.50.8650.8980.882 6.251.5441.5321.538 3.1252.0832.0982.091 1.5632.4692.4662.468 02.8442.8782.861 | |||||||
货期：3-5 working days
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