首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胎盘碱性磷酸酶(PLAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ALPP含量。ALPP 即烯醇化酶相关蛋白，目前有研究表明它与肿瘤发生发展相关，比如在一些肿瘤组织中呈现异常表达。其研究机制主要聚焦于它在细胞内信号传导、调控肿瘤细胞增殖、迁移和侵袭等过程中所扮演的角色，有望成为肿瘤诊断和治疗的新靶点。试剂盒检测范围为84 pg/mL-2000 pg/mL，该产品可为研究胎盘功能、胚胎发育调控、肿瘤微环境等领域的科研人员提供可靠工具，用于探索PLAP在生理或病理状态下的表达规律、评估疾病模型中的生物标志物变化，或筛选靶向PLAP的调控化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALPP; PLAP; Alkaline phosphatase, placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
缩写：ALPP
Uniprot No.：P05187
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：84 pg/mL-2000 pg/mL
灵敏度：34 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Tags & Cell Markers
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 88-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-104 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.0121.9831.9981.952 10001.3381.3411.3401.294 5000.7750.8280.8020.756 1670.3570.3490.3530.307 840.2240.2350.2300.184 00.0470.0450.046 | ||||||
货期：3-5 working days
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