首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠丙氨酸转氨酶/谷丙转氨酶(ALT/GPT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的ALT/GPT含量。ALT/GPT即谷丙转氨酶，主要存在于肝细胞内。肝细胞受损时其释放入血，致血清ALT/GPT水平升高，是反映肝细胞损伤的敏感指标。临床常通过检测其活性辅助诊断肝脏疾病，如肝炎、肝硬化等，研究也围绕其在疾病中的变化机制展开。试剂盒检测范围为0.156 mU/mL-10 mU/mL，广泛应用于肝损伤模型评估、药物肝毒性研究、代谢性疾病机制探索等科研领域，特别适用于体外培养肝细胞或动物模型中肝脏相关病理生理变化的动态监测，为肝脏功能研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
缩写：ALT/GPT
Uniprot No.：Q8QZR5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：0.156 mU/mL-10 mU/mL
灵敏度：0.039 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 98-113 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 102 | 95-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected102.6452.5662.6062.42152.0151.8551.9351.7502.51.1941.1551.1750.9901.250.7770.7960.7870.6020.6250.4920.4610.4770.2920.3120.3350.3440.3400.1550.1560.2780.2880.2830.09800.1910.1780.185 | ||||||
货期：3-5 working days
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