首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠花生四烯酸5脂氧合酶(Alox5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Alox5含量。Alox5即5-脂氧合酶，是催化花生四烯酸生成白三烯的关键酶。其白三烯在炎症、过敏等病理过程中作用显著。研究机制上，聚焦于抑制Alox5活性，以减少白三烯合成，为开发抗炎、抗过敏药物提供重要靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域研究Alox5在哮喘、关节炎、心血管疾病等病理模型中的表达变化，也可用于体外药物筛选或炎症相关信号通路机制探索，通过检测不同生物样本中的Alox5浓度，为疾病机制研究和潜在治疗靶点验证提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alox5Arachidonate 5-lipoxygenase ELISA Kit; 5-LO ELISA Kit; 5-lipoxygenase ELISA Kit; EC 1.13.11.34 ELISA Kit
缩写：Alox5
Uniprot No.：P12527
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Alox5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 95-106 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
回收率：
| The recovery of rat Alox5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 84-95 | |||||
| EDTA plasma (n=4) | 100 | 92-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.8172.7432.7802.666 52.3692.3042.3372.223 2.51.5741.5211.5481.434 1.250.8250.7890.8070.693 0.6250.4180.4020.4100.296 0.3120.2340.2280.2310.117 0.1560.1610.1650.1630.049 00.1130.1150.114 | |||||||
货期：3-5 working days
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