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产品描述：人花生四烯酸12 - 脂氧合酶，12S型(ALOX12/LOG12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的ALOX12含量。ALOX12即12-脂氧合酶，与花生四烯酸代谢有关。其它在多种生理和病理过程中起作用。研究机制上，主要涉及催化花生四烯酸生成12-氢过氧化二十碳四烯酸，在细胞增殖、炎症反应、肿瘤发生等方面有重要研究价值。试剂盒检测范围为1.25 ng/mL-80 ng/mL，本试剂盒通过预包被特异性捕获抗体与标记检测抗体的协同作用，可精准量化各类生物样本中的目标蛋白，为研究脂氧合酶家族功能、炎症信号通路调控、代谢性疾病机制等基础科研提供可靠工具，尤其适用于体外实验模型中的酶活性关联分析、药物干预效果评估及分子生物学机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：12 LOX ELISA Kit; 12(S) lipoxygenase ELISA Kit; 12-lipoxygenase ELISA Kit; 12LO ELISA Kit; 12S LOX ELISA Kit; 12S-lipoxygenase ELISA Kit; 12S-LOX ELISA Kit; 12S-type ELISA Kit; Alox12 ELISA Kit; Arachidonate 12 lipoxygenase ELISA Kit; Arachidonate 12 lipoxygenase, 12S type ELISA Kit; Arachidonate 12-lipoxygenase ELISA Kit; Arachidonate 12-oxidoreductase ELISA Kit; Lipoxin synthase 12-LO ELISA Kit; LOG12 ELISA Kit; LOX12_HUMAN ELISA Kit; P-12LO ELISA Kit; Platelet 12-LOX ELISA Kit; Platelet type lipoxygenase 12 ELISA Kit; platelet-type 12-lipoxygenase ELISA Kit; Platelet-type lipoxygenase 12 ELISA Kit
缩写：ALOX12
Uniprot No.：P18054
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ALOX12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-94 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human ALOX12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 802.4022.4552.4292.322 401.5991.6841.6421.535 200.8140.9020.8580.751 100.5050.5460.5260.419 50.3520.3670.3600.253 2.50.2460.2590.2530.146 1.250.1740.1770.1760.069 00.1030.1100.107 | ||||||
货期：3-5 working days
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