首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠乙醛脱氢酶(ALDH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ALDH2含量。ALDH2 即乙醛脱氢酶 2，在酒精代谢等生理过程中发挥关键作用。其基因多态性会影响酶活性。研究机制聚焦于其活性调节、与疾病关联等，如活性降低可致乙醛蓄积，增加多种疾病风险，相关研究为防治疾病提供新方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于酒精代谢机制研究、肝脏生理功能评估、抗氧化剂开发等科研领域，尤其适合检测微量样本中的ALDH表达动态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aldh2 ELISA Kit; Aldehyde dehydrogenase ELISA Kit; mitochondrial ELISA Kit; EC 1.2.1.3 ELISA Kit; ALDH class 2 ELISA Kit; ALDH-E2 ELISA Kit; ALDH1 ELISA Kit
缩写：ALDH2
Uniprot No.：P11884
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 95-102 | |||||
回收率：
| The recovery of rat ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-95 | ||||
| EDTA plasma (n=4) | 91 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7642.7532.7592.560102.1592.0422.1011.90251.5291.5121.5211.3222.51.0451.0181.0320.8331.250.6820.6560.6690.4700.6250.4230.4100.4170.2180.3120.3120.3030.3080.10900.2010.1970.199 | ||||||
货期：3-5 working days
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