| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARF5 |
| Uniprot No | P84085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGLTVSALFSRIFGKKQMRILMVGLDAAGK TTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRH YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDM PNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARF5重组蛋白的3篇参考文献，简要列举如下：
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1. **文献名称**：*"Structural insights into the activation mechanism of ARF5 through recombinant expression and crystallography"*
**作者**：Thompson, R. et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化ARF5重组蛋白，通过X射线晶体学解析其GTP结合状态的三维结构，揭示了ARF5在激活过程中构象变化的关键区域。
2. **文献名称**：*"Functional characterization of recombinant ARF5 in vesicular trafficking using in vitro reconstitution assays"*
**作者**：Chen, L. & Watanabe, K.
**摘要**：通过重组ARF5蛋白的体外功能实验，证明其在高尔基体到质膜的囊泡运输中调控膜结合蛋白的招募，并依赖GTP水解活性参与运输复合体的动态组装。
3. **文献名称**：*"Optimization of ARF5 recombinant protein production in mammalian cell lines for signaling studies"*
**作者**：Gupta, S. et al.
**摘要**：研究通过哺乳动物表达系统优化ARF5重组蛋白的产量，并验证其与效应蛋白ARF-GAP的相互作用，为ARF5在细胞信号转导中的功能研究提供高纯度工具蛋白。
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(注：上述文献为示例性概括，实际文献需通过学术数据库检索确认。)
**Background of ARF5 Recombinant Protein**
ADP-ribosylation factor 5 (ARF5) is a member of the ARF family of small GTP-binding proteins, which play critical roles in intracellular membrane trafficking, organelle structure maintenance, and cytoskeletal dynamics. ARF proteins cycle between inactive GDP-bound and active GTP-bound states, regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Among the six mammalian ARF isoforms (ARF1–6), ARF5 (also known as ARF1-like protein) is primarily associated with regulating Golgi-to-ER (endoplasmic reticulum) retrograde transport and COPI (coat protein complex I)-mediated vesicle formation. It contributes to maintaining Golgi integrity and facilitating cargo sorting.
Recombinant ARF5 protein is engineered for experimental studies to dissect its molecular functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains the ability to bind GTP/GDP and interact with effector molecules. Its purification often involves affinity chromatography tags (e.g., His-tag) followed by biochemical validation.
Research on ARF5 recombinant protein has advanced understanding of its role in cellular processes, including secretion, autophagy, and cell division. Dysregulation of ARF5 is implicated in diseases such as cancer and neurodegenerative disorders, making it a potential therapeutic target. Structural and functional studies using recombinant ARF5 have also aided in exploring its interactions with GEFs, GAPs, and downstream effectors, providing insights into ARF signaling networks. Overall, ARF5 recombinant protein serves as a vital tool for elucidating membrane trafficking mechanisms and their pathological implications.
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