首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人芳香烃受体(AhR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AHR含量。AHR即芳香烃受体，在环境应激反应、免疫调节及细胞生长分化等方面起重要作用。研究机制上，它能与多种配体结合，激活后转位至细胞核，调控下游基因表达，参与多种生理和病理过程，如炎症、肿瘤发生发展等。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中AhR相关分子机制研究，例如环境污染物（如二噁英）暴露后AhR信号通路激活分析、免疫细胞功能调控探索，或肿瘤、炎症性疾病模型中AhR表达动态监测，为分子生物学及药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ah receptor ELISA Kit; AhR ELISA Kit; AHR_HUMAN ELISA Kit; Aromatic hydrocarbon receptor ELISA Kit; Aryl hydrocarbon receptor ELISA Kit; Aryl hydrocarbon receptor precursor ELISA Kit; bHLHe76 ELISA Kit; Class E basic helix loop helix protein 76 ELISA Kit; Class E basic helix-loop-helix protein 76 ELISA Kit; HGNC:348 ELISA Kit
缩写：AHR
Uniprot No.：P35869
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AhR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human AhR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.2312.1082.1701.988 5001.7941.7621.7781.596 2501.3661.3671.3671.185 1250.8610.8980.8800.698 62.50.6320.6020.6170.435 31.20.4810.4720.4770.295 15.60.3570.3420.3500.168 00.1810.1830.182 | ||||||
货期：3-5 working days
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