首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血管紧张素原(aGT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGT含量。AGT即血管紧张素原，它是肾素-血管紧张素系统（RAS）的唯一底物。其背景在于它对血压调节等生理过程意义重大。研究机制方面，肾素会作用于AGT使其裂解，生成血管紧张素I，再经血管紧张素转换酶作用生成血管紧张素II，进而影响血管收缩等。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于心血管疾病动物模型研究、降压药物作用机制探索或病理状态下RAS系统活性评估等科研场景，为研究肾素 - 血管紧张素通路相关分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Agt ELISA Kit; Serpina8Angiotensinogen ELISA Kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA Kit; Angiotensin 1-10 ELISA Kit; Angiotensin I ELISA Kit; Ang I); Angiotensin-2 ELISA Kit; Angiotensin 1-8 ELISA Kit; Angiotensin II ELISA Kit; Ang II); Angiotensin-3 ELISA Kit; Angiotensin 2-8 ELISA Kit; Angiotensin III ELISA Kit; Ang III ELISA Kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA Kit; Angiotensin 3-8 ELISA Kit; Angiotensin IV ELISA Kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA Kit
缩写：AGT
Uniprot No.：P01015
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 107 | |||||
| Range % | 100-111 | ||||||
| 1:200 | Average % | 104 | |||||
| Range % | 97-108 | ||||||
| 1:400 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:800 | Average % | 94 | |||||
| Range % | 85-97 | ||||||
回收率：
| The recovery of rat aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-95 | |||||
| EDTA plasma (n=4) | 101 | 93-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.2042.0992.1522.015 10001.7441.7131.7291.592 5001.0981.0671.0830.946 2500.6050.6170.6110.474 1250.4310.4210.4260.289 62.50.2770.2660.2720.135 31.250.1750.1810.1780.041 00.1380.1360.137 | |||||||
货期：3-5 working days
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