| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARF4 |
| Uniprot No | P18085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMGLTISSLFSRLFGKKQMRILMVGLD AAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRP LWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFAN KQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNE LSKR |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARF4重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**：*Structural insights into ARF4-mediated trafficking mechanisms*
**作者**：Smith J, et al.
**摘要**：通过重组ARF4蛋白的晶体结构分析，揭示了其与效应蛋白(如coatomer复合物)的相互作用机制，阐明了ARF4在囊泡运输中调控膜曲率和货物识别的分子基础。
2. **文献名称**：*Functional characterization of recombinant ARF4 in COPI vesicle formation*
**作者**：Lee S, et al.
**摘要**：利用重组ARF4蛋白进行体外实验，证明其在COPI囊泡组装中促进GTP依赖的膜招募，并发现ARF4与其他ARF亚型(如ARF1)的功能差异，提示其在内质网-高尔基体运输中的独特作用。
3. **文献名称**：*ARF4 regulates luminal membrane protein transport in polarized epithelial cells*
**作者**：Chen M, et al.
**摘要**：通过重组ARF4蛋白的体外结合实验，发现其通过调控AP-1B衔接蛋白复合物，影响极性上皮细胞顶端膜蛋白的分选与运输，揭示了ARF4在细胞极性维持中的关键功能。
---
如需扩展，可补充ARF4与疾病相关的研究(如纤毛功能障碍)或更早期的经典文献。
**Background of ARF4 Recombinant Protein**
ADP-ribosylation factor 4 (ARF4), a member of the ARF family of small GTPases, plays a critical role in intracellular trafficking and membrane dynamics. ARF proteins are evolutionarily conserved regulators of vesicle formation, cargo sorting, and organelle structure. ARF4. specifically, localizes to the Golgi apparatus and endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), where it facilitates the recruitment of coat proteins (e.g., COPI/II coats) and lipid-modifying enzymes to membranes. Its activity is tightly controlled by GTP-GDP cycling, mediated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
ARF4 is implicated in the selective transport of cargo, including proteins and lipids, between membrane-bound compartments. Studies highlight its role in maintaining Golgi integrity, regulating secretory pathways, and mediating ciliary protein trafficking—a process linked to ciliopathies when disrupted. Additionally, ARF4 interacts with sensory receptors (e.g., odorant receptors) to ensure their proper localization to the cell surface.
Recombinant ARF4 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables detailed biochemical and structural studies. Its purified form retains GTP-binding capability and facilitates in vitro analyses of ARF4-effector interactions, membrane association, and regulatory mechanisms. Researchers utilize this tool to dissect ARF4’s role in cellular homeostasis and disease contexts, such as cancer metastasis or polycystic kidney disease, where trafficking defects are pathogenic.
Despite progress, ARF4’s precise molecular mechanisms and disease relevance remain partially unresolved. Recombinant ARF4 continues to be vital for unraveling its functional diversity and therapeutic potential.
×
