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Human proteoglycan 4(PRG4) ELISA Kit

  • 中文名： 人蛋白聚糖4(PRG4) ELISA Kit
货号: IPD-E14124h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人蛋白聚糖4(PRG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的PRG4含量。试剂盒检测范围为0.78 ng/ml-50 ng/ml，灵敏度为0.195 ng/ml。该产品适用于关节炎机制研究、药物开发中生物标志物分析，或体外模型中 PRG4 表达水平的动态监测，为探索关节退行性疾病病理机制及潜在治疗靶点提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    PRG4 ELISA Kit; MSF ELISA Kit; SZP ELISA Kit; Proteoglycan 4 ELISA Kit; Lubricin ELISA Kit; Megakaryocyte-stimulating factor ELISA Kit; Superficial zone proteoglycan) [Cleaved into: Proteoglycan 4 C-terminal part] ELISA Kit
  • 缩写：
    PRG4
  • Uniprot No.：
    Q92954
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma and tissue homogenates.
  • 检测范围：
    0.78 ng/ml-50 ng/ml.
  • 灵敏度：
    0.195 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    109


    Range %

    102-115


    1:2

    Average %

    100


    Range %

    95-106


    1:4

    Average %

    94


    Range %

    89-100


    1:8

    Average %

    101


    Range %

    96-107


  • 回收率：

    The recovery of human PRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    100

    96-106


    EDTA plasma (n=4)

    96

    90-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.524

    2.634

    2.579

    2.406


    25

    1.902

    1.967

    1.934

    1.762


    12.5

    1.286

    1.228

    1.257

    1.084


    6.25

    0.840

    0.829

    0.835

    0.662


    3.12

    0.476

    0.451

    0.464

    0.291


    1.56

    0.385

    0.373

    0.379

    0.206


    0.78

    0.264

    0.274

    0.269

    0.096


    0

    0.170

    0.175

    0.173



  • 本试剂盒所含材料：


    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PRG4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled PRG4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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