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Rat neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

  • 中文名： 大鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA Kit
货号: IPD-E09409r
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    大鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LCN2含量。LCN2，即脂质运载蛋白2，也称为NGAL，是一种在多种细胞中表达的分泌蛋白，负责运输亲脂小分子。它在多种疾病中发挥作用，包括癌症、中枢神经系统疾病、代谢性疾病和肾损伤。LCN2通过调节铁的运输、影响信号通路和参与炎症反应等机制，在疾病的发生发展中扮演重要角色。试剂盒检测范围为0.3125 ng/ml- 20ng/ml，灵敏度为0.078 ng/ml。适用于科研领域中对肾脏病理模型、药物干预效果或炎症相关分子机制的研究，可兼容血清、血浆等多种液态样本及肾脏、肝脏等组织匀浆样本检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Lcn2 ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; Alpha-2-microglobulin-related protein ELISA Kit; Alpha-2U globulin-related protein ELISA Kit; Lipocalin 24p3 ELISA Kit; Lipocalin-2 ELISA Kit; Siderocalin LCN2 ELISA Kit; p25 ELISA Kit
  • 缩写：
    LCN2
  • Uniprot No.：
    P30152
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/ml-20 ng/ml.
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    80-98


    1:2

    Average %

    95


    Range %

    89-99


    1:4

    Average %

    85


    Range %

    81-90


    1:8

    Average %

    104


    Range %

    99-107


  • 回收率：

    The recovery of rat NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    93-104


    EDTA plasma (n=4)

    95

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.451

    2.498

    2.475

    2.287


    10

    1.954

    1.967

    1.961

    1.773


    5

    1.226

    1.269

    1.248

    1.060


    2.5

    0.758

    0.789

    0.774

    0.586


    1.25

    0.529

    0.505

    0.517

    0.329


    0.625

    0.380

    0.351

    0.366

    0.178


    0.312

    0.279

    0.271

    0.275

    0.087


    0

    0.187

    0.189

    0.188



  • 本试剂盒所含材料：


    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat NGAL. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled NGAL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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