首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒检测血清、血浆、尿液、组织匀浆样本中的NGAL含量。NGAL即中性粒细胞明胶酶相关脂质运载蛋白，在多种生理和病理过程中发挥重要作用。其表达异常与肿瘤、肾脏疾病等密切相关。研究机制主要聚焦于其生物学功能及信号通路，如参与铁代谢、炎症反应等，有望成为疾病诊断和治疗的新靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于体外培养细胞模型、动物实验样本或临床前研究中的NGAL动态监测；可为急性肾损伤模型评估、炎症相关疾病研究以及生物标志物筛选提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：24p3 ELISA Kit; 25 kDa alpha 2 microglobulin related subunit of MMP9 ELISA Kit; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA Kit; Alpha 2 microglobulin related protein ELISA Kit; HGNC:6526 ELISA Kit; HNL ELISA Kit; Lcn 2 ELISA Kit; Lcn2 ELISA Kit; Lipocalin-2 ELISA Kit; Migration stimulating factor inhibitor ELISA Kit; MSFI ELISA Kit; Neutrophil gelatinase associated lipocalin ELISA Kit; Neutrophil gelatinase associated lipocalin precursor ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; NGAL_HUMAN ELISA Kit; Oncogene 24p3 ELISA Kit; Oncogenic lipocalin 24p3 ELISA Kit; p25 ELISA Kit; Siderocalin ELISA Kit; siderocalin LCN2 ELISA Kit; SV40 induced 24P3 protein ELISA Kit; Uterocalin ELISA Kit
缩写：NGAL
Uniprot No.：P80188
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.1402.1362.1381.988 5001.8241.8251.8251.675 2501.3671.3321.3501.200 1250.9791.0231.0010.851 62.50.5100.5510.5310.381 31.20.3550.3720.3640.214 15.60.2710.2890.2800.130 00.1500.1490.150 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NGAL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NGAL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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