| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NREP |
| Uniprot No | Q16612 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-68aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMVYYPELFVWVSQEPFPNKDMEGRLPK GRLPVPKEVNRKKNDETNAASLTPLGSSELRSPRISYLHFF |
| 预测分子量 | 10 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NREP(Neuronal Regeneration-Related Protein)重组蛋白的虚构参考文献示例，涵盖不同研究方向：
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1. **文献名称**：*"Recombinant Expression and Functional Characterization of NREP in Axonal Regeneration Models"*
**作者**：Chen L., et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了高纯度NREP重组蛋白，并通过体外神经元培养模型证实其能显著促进轴突生长。Western blot和免疫荧光显示NREP通过激活PI3K/Akt信号通路增强神经元存活率，为神经损伤修复提供了潜在治疗靶点。
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2. **文献名称**：*"NREP Knockdown Exacerbates Ischemic Stroke Damage via ER Stress Modulation"*
**作者**：Smith J.R., et al.
**摘要**：通过腺病毒载体构建重组NREP蛋白，研究发现其在脑缺血再灌注小鼠模型中能减轻神经元凋亡。机制研究表明，NREP通过抑制内质网应激相关蛋白(如CHOP和GRP78)的表达，降低氧化应激水平，改善神经功能缺损评分。
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3. **文献名称**：*"Structural Insights into NREP’s Heparin-Binding Domain for Neurotrophic Activity"*
**作者**：Yamamoto K., et al.
**摘要**：采用X射线晶体学解析了NREP重组蛋白的Heparin结合域三维结构，揭示其通过静电相互作用与细胞外基质结合。体外实验表明，该结构域突变体会显著削弱NREP对背根神经节神经元的神经营养作用，为设计靶向神经再生药物提供了结构基础。
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4. **文献名称**：*"NREP Recombinant Protein Attenuates Neuroinflammation in Alzheimer’s Disease Mice"*
**作者**：Gomez M.A., et al.
**摘要**：在APP/PS1转基因小鼠中，腹腔注射NREP重组蛋白可降低脑内Aβ沉积和胶质细胞活化。RNA-seq分析表明，NREP通过调控TREM2/DAP12通路抑制神经炎症反应，改善认知功能，提示其在神经退行性疾病中的治疗潜力。
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注：以上文献为示例性内容，实际研究中请以真实发表的论文为准。如需查找真实文献，建议通过PubMed或Web of Science以“NREP recombinant protein”或“Neuronal regeneration protein”为关键词检索。
**Background of NREP Recombinant Protein**
NREP (Neuron-Derived Neurotrophic Factor-Related Protein), also known as Neuritin 1 (NRN1), is a small, secreted glycoprotein initially identified for its role in neural development and synaptic plasticity. It belongs to the neuritin family and is highly conserved across species, underscoring its functional significance. NREP is predominantly expressed in the nervous system, particularly in neurons, where it promotes neurite outgrowth, dendritic arborization, and synaptic maturation. Its expression is regulated by neuronal activity and neurotrophic factors, such as brain-derived neurotrophic factor (BDNF), linking it to mechanisms of learning, memory, and neural repair.
Structurally, NREP contains a glycosylphosphatidylinositol (GPI) anchor motif, enabling membrane association, and a conserved cysteine-rich domain critical for its neurotrophic activity. Recombinant NREP is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. Studies highlight its therapeutic potential in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s) and nerve injury recovery by enhancing neuronal survival and regeneration.
Beyond neurology, NREP exhibits roles in non-neural tissues, including angiogenesis and immune modulation, suggesting broader physiological impacts. Its recombinant form is pivotal in dissecting molecular pathways and developing targeted therapies, making it a focal point in both basic and translational neuroscience research.
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