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产品描述：绵羊花生四烯酸(AA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的AA含量。试剂盒检测范围为15.625 ng/mL-1000 ng/mL，灵敏度为13.797 ng/mL。该产品适用于科研领域中对绵羊模型炎症机制、营养代谢调控、药物干预效果的评价，以及脂质代谢相关通路的基础研究，为体外实验提供可靠的定量数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AA
种属：Ovis aries (Sheep)
样本类型：serum, plasma
检测范围：15.625 ng/mL-1000 ng/mL
灵敏度：13.797 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 89-100 | ||||||
| 1:200 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:400 | Average % | 100 | |||||
| Range % | 94-104 | ||||||
| 1:800 | Average % | 105 | |||||
| Range % | 97-110 | ||||||
回收率：
| The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 95-106 | |||||
| EDTA plasma (n=4) | 102 | 96-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 10000.1220.1200.121 5000.1660.1580.162 2500.2320.2410.237 1250.3370.3440.341 62.50.5280.5100.519 31.250.9410.9550.948 15.6251.4711.5221.497 02.3262.4192.373 | |||||||
货期：3-5 working days
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