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产品描述：人白介素16(IL-16)酶联免疫试剂盒 为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL16含量。IL16是一种多功能细胞因子，主要在T淋巴细胞产生，具有调节免疫和炎症的功能。它通过直接结合CD4，诱导T细胞和单核细胞迁移，参与炎症反应和免疫调节。研究IL16有助于理解免疫反应，并开发针对炎症和自身免疫性疾病的靶向疗法。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。适用于体外培养细胞分泌检测、动物模型组织表达分析等科研场景，尤其适合研究感染性疾病、自身免疫疾病或肿瘤微环境中IL - 16的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HGNC:5980 ELISA Kit; HsT19289 ELISA Kit; IL-16 ELISA Kit; IL16 ELISA Kit; IL16_HUMAN ELISA Kit; Interleukin 16 precursor ELISA Kit; Interleukin-16 ELISA Kit; LCF ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; Neuronal interleukin 16 ELISA Kit; NIL16 ELISA Kit; prIL 16 ELISA Kit; PrIL16 ELISA Kit; Prointerleukin 16 ELISA Kit
缩写：IL16
Uniprot No.：Q14005
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-104 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 81-88 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 81-96 | ||||||
回收率：
| The recovery of human IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 94-101 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 101.7741.8481.8111.644 51.0031.0141.0090.842 2.50.6660.6870.6770.510 1.250.4010.4120.4070.240 0.6250.2890.2870.2880.121 0.3120.2450.2530.2490.082 0.1560.2110.2240.2180.051 00.1630.1700.167 | |||||||
货期：3-5 working days
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