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产品描述：鸡卵黄免疫球蛋白(IgY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、蛋黄样本中的IgY含量。IgY即免疫球蛋白Y，是禽类血清和卵黄中的主要抗体。其背景在于可从卵黄高效提取，成本低、产量大。研究机制方面，IgY能特异性结合抗原，在抗感染、免疫诊断等领域展现作用，如中和病原体、阻断其与宿主细胞结合等。试剂盒检测范围为0.078 ng/mL-5 ng/mL，灵敏度为0.02 ng/mL。该产品可广泛应用于家禽免疫学研究、疫苗免疫效果评估、抗体生产工艺优化及生物制品质量控制等科研场景，为禽类相关疾病模型的免疫指标分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgY
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, egg yolk
检测范围：0.078 ng/mL-5 ng/mL
灵敏度：0.02 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:500000 | Average % | 89 | ||||
| Range % | 81-100 | |||||
| 1:1000000 | Average % | 96 | ||||
| Range % | 87-103 | |||||
| 1:2000000 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4000000 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-105 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 52.7812.9112.8462.737 2.52.3162.3422.3292.220 1.251.5791.5841.5821.473 0.6250.9010.8830.8920.783 0.3120.4430.4360.4400.331 0.1560.3150.3190.3170.208 0.0780.2490.2470.2480.139 00.1080.1100.109 | ||||||
货期：3-5 working days
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