Rat Advanced glycosylation end product-specific receptor(AGER) ELISA kit

中文名： 大鼠晚期糖基化终产物特异性受体(AGER)ELISA kit

货号: IPD-EL001441RA

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
大鼠晚期糖基化终产物特异性受体(AGER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的AGER含量。AGER即晚期糖基化终末产物受体，在炎症、氧化应激等病理过程发挥重要作用。研究显示，它可与多种配体结合，激活下游信号通路，引发细胞内一系列反应，参与糖尿病并发症、神经退行性疾病、肿瘤等疾病发生发展，是潜在治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.81 pg/mL。该产品主要应用于基础研究领域，如糖尿病相关器官损伤的分子机制探索、神经炎症模型的蛋白表达分析，或药物干预下AGER信号通路变化的体外评估，为科研人员提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Ager ELISA Kit; Rage ELISA Kit; Advanced glycosylation end product-specific receptor ELISA Kit; Receptor for advanced glycosylation end products ELISA Kit
缩写：
AGER
Uniprot No.：
Q63495
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma,tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.81 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)224.423224.423225.339224.377225.431224.881SD0.0340.0300.0310.0400.0370.039CV(%)4.4333.9114.0315.2164.8105.078

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AGER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

90-94

1:2

Average %

Range %

90-102

1:4

Average %

102

Range %

99-105

1:8

Average %

Range %

80-94

回收率：

The recovery of rat AGER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	97-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
2000	2.703	2.878	2.791	2.615
1000	1.903	1.856	1.880	1.704
500	1.245	1.154	1.200	1.024
250	0.731	0.747	0.739	0.563
125	0.556	0.533	0.545	0.369
62.5	0.402	0.392	0.397	0.221
31.25	0.272	0.275	0.274	0.098
0	0.173	0.179	0.176