Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

中文名： 大鼠上皮中性粒细胞活化肽78(ENA-78/CXCL5)ELISA Kit

货号: IPD-E08179r

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
大鼠上皮中性粒细胞活化肽78(ENA-78/CXCL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL5含量。CXCL5 属于趋化因子，在炎症反应、肿瘤发展等过程中有重要作用。其研究机制主要涉及与受体结合，激活下游信号通路，促进细胞迁移、增殖等。在肿瘤领域，它可影响肿瘤微环境，促进血管生成，为相关疾病治疗提供潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。本试剂盒适用于科研领域中对CXCL5在炎症模型、免疫调节或疾病机制研究中的动态变化分析，如通过血清或血浆样本评估系统性炎症状态，或利用组织匀浆探究局部CXCL5表达特征，为探索相关信号通路、药物干预效果等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5 ELISA Kit
缩写：
CXCL5
Uniprot No.：
P97885
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	2.442	2.474	2.529	2.446	2.487	2.542
SD	0.035	0.033	0.038	0.041	0.051	0.048
CV(%)	5.486	5.116	5.784	6.416	7.870	7.273

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

83-91

1:2

Average %

104

Range %

98-108

1:4

Average %

Range %

87-96

1:8

Average %

Range %

91-99

回收率：

The recovery of rat ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	94	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
20	2.746	2.689	2.718	2.607
10	2.000	1.899	1.950	1.839
5	1.190	1.171	1.181	1.070
2.5	0.651	0.640	0.646	0.535
1.25	0.366	0.354	0.360	0.249
0.625	0.263	0.257	0.260	0.149
0.312	0.172	0.178	0.175	0.064	0.064
0	0.110	0.112	0.111