Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit_人ELISA试剂盒_ELISA试剂盒_产品

Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit

货号: IPD-E04911h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人抗双链DNA(dsDNA)抗体/天然DNA抗体(IgG)酶联免疫试剂盒为间接法ELISA试剂盒，定性检测血清样本中的dsDNA Ab (IgG)含量。dsDNA Ab (IgG)即抗双链 DNA 抗体（IgG 型），其产生与机体自身免疫紊乱有关，常见于系统性红斑狼疮等自身免疫病。研究认为它可与体内双链 DNA 结合形成免疫复合物，沉积于组织引发炎症反应，干扰正常生理功能，是相关疾病重要研究靶点。本产品适用于科研场景中SLE相关抗体水平分析、疾病活动度关联研究，或用于评估实验性治疗对自身免疫反应的影响，为免疫病理学研究及药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
N/A
缩写：
dsDNA Ab (IgG)
种属：
Homo sapiens (Human)
样本类型：
serum
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Autoimmunity
测定原理：
qualitative
测定方法：
Indirect

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

A micro ELISA plate --- The 96-well plate has been pre-coated with antigen-specific for dsDNA Ab (IgG). This dismountable microplate can be divided into 12 x 8 strip plates.
Negative Control (1 x 800 μl)
Positive Control (1 x 800 μl)
HRP-conjugate (1 x10 ml) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Sample Diluent (1 x 20 ml) --- Dilute the standard and sample solution.
Wash Buffer (25 x concentrate) (1 x20 ml) --- Wash away unbound or free substances.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate