Recombinant Human NDRG2 protein

中文名： N-myc下游调节基因2(NDRG2)重组蛋白
别名： NDRG2;KIAA1248;SYLD;Protein NDRG2

货号: PA1000-2112

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	NDRG2
Uniprot No	Q9UN36
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-357aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSHMAELQE VQITEEKPLL PGQTPEAAKT HSVETPYGSV TFTVYGTPKP KRPAILTYHD VGLNYKSCFQ PLFQFEDMQE IIQNFVRVHV DAPGMEEGAP VFPLGYQYPS LDQLADMIPC VLQYLNFSTI IGVGVGAGAY ILARYALNHP DTVEGLVLIN IDPNAKGWMD WAAHKLTGLT SSIPEMILGH LFSQEELSGN SELIQKYRNI ITHAPNLDNI ELYWNSYNNR RDLNFERGGD ITLRCPVMLV VGDQAPHEDA VVECNSKLDP TQTSFLKMAD SGGQPQLTQP GKLTEAFKYF LQGMGYMASS CMTRLSRSRT ASLTSAASVD GNRSRSRTLS QSSESGTLSS GPPGHTMEVS C
预测分子量	42 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

1. **《Expression and purification of recombinant human NDRG2 protein in Escherichia coli》**

作者：Zhang Y, et al.

摘要：该研究建立了人源NDRG2蛋白在大肠杆菌中的高效可溶性表达系统，通过His标签纯化获得高纯度蛋白，并验证了其与金属离子的结合特性。

2. **《Functional characterization of recombinant NDRG2 in cell differentiation》**

作者：Liu X, et al.

摘要：利用哺乳动物细胞表达系统制备重组NDRG2.证明其通过调控TGF-β信号通路促进神经细胞分化，为神经退行性疾病研究提供工具蛋白。

3. **《NDRG2 recombinant protein inhibits tumor growth via PI3K/AKT pathway suppression》**

作者：Wang L, et al.

摘要：通过杆状病毒系统表达NDRG2重组蛋白，发现其通过抑制PI3K/AKT通路降低肝癌细胞增殖，提示潜在抗肿瘤治疗应用。

4. **《Structural analysis of NDRG2 recombinant protein using cryo-EM》**

作者：Chen H, et al.

摘要：首次解析了重组NDRG2蛋白的冷冻电镜三维结构，揭示其α/β折叠核心域与磷酸化修饰位点，为功能机制研究提供结构基础。

背景信息

**Background of NDRG2 Recombinant Protein**

NDRG2 (N-Myc downstream-regulated gene 2) is a member of the NDRG family, a group of evolutionarily conserved proteins implicated in cell differentiation, stress responses, and tumor suppression. Unlike other family members (NDRG1. NDRG3. and NDRG4), NDRG2 is widely expressed in various tissues, including the brain, liver, kidney, and skeletal muscle, and plays diverse roles in cellular processes such as proliferation, apoptosis, and metabolism. Structurally, NDRG2 is a 40-45 kDa cytoplasmic protein containing an α/β-hydrolase-fold domain, though its enzymatic activity remains unclear.

NDRG2 is recognized as a tumor suppressor, with its downregulation linked to cancers like glioma, hepatocellular carcinoma, and breast cancer. It regulates cell cycle progression by modulating key signaling pathways, including PI3K/AKT, MAPK, and Wnt/β-catenin. Epigenetic mechanisms, such as promoter methylation and histone modification, often silence NDRG2 expression in malignancies. Beyond oncology, NDRG2 participates in stress responses, interacting with hypoxia-inducible factor 1α (HIF-1α) and p53 to mediate cellular adaptation to hypoxia and oxidative stress.

In the nervous system, NDRG2 is critical for maintaining glial cell function and neuronal survival. It is upregulated in neurodegenerative disorders like Alzheimer’s disease and multiple sclerosis, suggesting roles in neuroinflammation and myelin regulation. Recombinant NDRG2 protein, produced via bacterial or mammalian expression systems, enables functional studies to dissect these mechanisms. Its applications span *in vitro* assays, animal models, and structural analyses, though post-translational modifications may require mammalian systems for accurate replication.

Overall, NDRG2 recombinant protein serves as a vital tool for exploring its dual roles in health and disease, offering potential as a diagnostic marker or therapeutic target.