首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血管内皮细胞生长因子(VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物、尿液样本中的VEGFA含量。VEGFA即血管内皮生长因子A，是一种促进血管生成的重要蛋白。其背景在于在肿瘤生长、转移及多种眼部疾病等病理过程中高表达。研究机制为与受体结合激活下游信号通路，促进内皮细胞增殖、迁移和血管生成，是抗血管生成治疗的关键靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为25.297 pg/mL。在肿瘤血管生成机制研究、心血管疾病模型构建、创伤修复实验等科研领域具有广泛应用价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：glioma-derived endothelial cell mitogen ELISA Kit; MGC70609 ELISA Kit; MVCD1 ELISA Kit; Vascular endothelial growth factor A ELISA Kit; Vascular Endothelial Growth Factor ELISA Kit; Vascular permeability factor ELISA Kit; VEGF ELISA Kit; VEGF-A ELISA Kit; VEGF165 ELISA Kit; Vegfa ELISA Kit; VEGFA_HUMAN ELISA Kit; VPF ELISA Kit
缩写：VEGFA
Uniprot No.：P15692
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：25.297 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.6512.7512.7012.547 10001.7291.8291.7791.625 5001.0671.0871.0770.923 2500.5790.5890.5840.430 1250.3720.3820.3770.223 62.50.2550.2650.2600.106 31.250.2070.2090.2080.054 00.1530.1540.154 | ||||||
货期：3-5 working days
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