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Human versican/PG-M/PG-350 ELISA kit

  • 中文名： 人多功能蛋白聚糖(versican)ELISA Kit
货号: IPD-E11884h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人多功能蛋白聚糖(versican)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的VCAN含量。VCAN即多功能蛋白聚糖，是细胞外基质的重要组成部分。它参与多种生理和病理过程，如细胞黏附、迁移等。在研究机制上，它通过与多种分子相互作用，影响细胞信号传导，在肿瘤、心血管疾病等研究领域受关注，为疾病诊断和治疗提供思路。试剂盒检测范围为0.04 ng/ml-30 ng/ml.，灵敏度为0.04 ng/ml.。该产品适用于心血管病理机制研究、肿瘤侵袭转移模型构建、软骨组织工程研究等基础科研领域，支持多种生物样本的检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Chondroitin sulfate proteoglycan 2 ELISA Kit; Chondroitin sulfate proteoglycan core protein 2 ELISA Kit; Chondroitin sulfate proteoglycan core protein; cartilage ELISA Kit; CSPG2 ELISA Kit; CSPG2_HUMAN ELISA Kit; ERVR ELISA Kit; GHAP ELISA Kit; Glial hyaluronate binding protein ELISA Kit; Glial hyaluronate-binding protein ELISA Kit; Large fibroblast proteoglycan ELISA Kit; PG-M ELISA Kit; PGM ELISA Kit; VCAN ELISA Kit; Versican ELISA Kit; Versican core protein ELISA Kit; Versican proteoglycan ELISA Kit; WGN 1 ELISA Kit; WGN ELISA Kit; WGN1 ELISA Kit
  • 缩写：
    VCAN
  • Uniprot No.：
    P13611
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.04 ng/ml-30 ng/ml.
  • 灵敏度：
    0.04 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human versican/PG-M/PG-350 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:20

    Average %

    90


    Range %

    85-95


    1:40

    Average %

    97


    Range %

    91-105


    1:80

    Average %

    92


    Range %

    88-97


    1:160

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human versican/PG-M/PG-350 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    90

    84-95


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    30

    2.803

    2.734

    2.768

    2.595


    10

    2.365

    2.367

    2.366

    2.193


    3.33

    1.805

    1.828

    1.816

    1.644


    1.11

    1.114

    1.129

    1.121

    0.948


    0.37

    0.673

    0.651

    0.662

    0.489


    0.12

    0.384

    0.363

    0.373

    0.200


    0.04

    0.265

    0.274

    0.269

    0.096


    0

    0.171

    0.175

    0.173




  • 货期：
    3-5 working days

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