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Human Thioredoxin domain-containing protein 5(TXNDC5) ELISA kit

  • 中文名： 人含硫氧还蛋白结构域蛋白5(TXNDC5)酶联免疫试剂盒
货号: IPD-EL025379HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人含硫氧还蛋白结构域蛋白5(TXNDC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TXNDC5含量。TXNDC5是一种位于内质网腔内的蛋白质二硫键异构酶，对蛋白质稳定性和功能至关重要。其研究机制涉及催化二硫键重排、减少异常蛋白质合成，具有抗氧化、抑制细胞凋亡等作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。可为科研人员探索TXNDC5在代谢性疾病、炎症反应或癌症发生发展中的分子机制提供可靠工具，亦可用于评估药物干预或基因修饰对TXNDC5表达水平的影响，为相关基础研究与生物标志物筛选提供数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    EndoPDI ELISA Kit; Endoplasmic reticulum protein ERp46 ELISA Kit; Endoplasmic reticulum resident protein 46 ELISA Kit; Endothelial protein disulphide isomerase ELISA Kit; ER protein 46 ELISA Kit; ERp46 ELISA Kit; Hcc 2 ELISA Kit; MGC3178 ELISA Kit; PDIA15 ELISA Kit; Protein disulfide isomerase family A member 15 ELISA Kit; STRF8 ELISA Kit; Thioredoxin domain containing 5 (endoplasmic reticulum) ELISA Kit; Thioredoxin domain containing 5 ELISA Kit; Thioredoxin domain containing protein 5 ELISA Kit; Thioredoxin domain-containing protein 5 ELISA Kit; Thioredoxin like protein p46 ELISA Kit; Thioredoxin related protein ELISA Kit; Thioredoxin-like protein p46 ELISA Kit; TLP46 ELISA Kit; TXND5_HUMAN ELISA Kit; TXNDC 5 ELISA Kit; Txndc5 ELISA Kit
  • 缩写：
    TXNDC5
  • Uniprot No.：
    Q8NBS9
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TXNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:5

    Average %

    90


    Range %

    87-94


    1:10

    Average %

    90


    Range %

    85-94


    1:20

    Average %

    88


    Range %

    80-96


    1:40

    Average %

    92


    Range %

    86-99


  • 回收率：

    The recovery of human TXNDC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    97

    89-104


    EDTA plasma (n=4)

    94

    90-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.286

    2.186

    2.236

    2.083


    5

    1.752

    1.652

    1.702

    1.549


    2.5

    1.193

    1.153

    1.173

    1.020


    1.25

    0.704

    0.694

    0.699

    0.546


    0.625

    0.441

    0.431

    0.436

    0.283


    0.312

    0.311

    0.301

    0.306

    0.153


    0.156

    0.236

    0.226

    0.231

    0.078


    0

    0.153

    0.152

    0.153




  • 货期：
    3-5 working days

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