首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human tumor necrosis factor-related apoptosis-inducing ligand receptor 3,TRAIL-R3 ELISA Kit

  • 中文名： 人肿瘤坏死因子相关凋亡诱导配体受体3(TRAIL-R3)ELISA Kit
货号: IPD-E04748h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人肿瘤坏死因子相关凋亡诱导配体受体3(TRAIL-R3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRAIL-R3含量。TRAIL-R3是重要的靶点。其它属于肿瘤坏死因子受体超家族成员。研究机制上，它可负调控TRAIL诱导的凋亡信号，在肿瘤细胞逃避凋亡中起作用，对其深入研究有助于了解肿瘤发生发展机制，为肿瘤治疗提供新靶点与思路。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于基础科研领域，例如探究肿瘤发生机制、细胞凋亡信号通路调控等研究场景，可为体外分析不同生物样本中TRAIL-R3的蛋白表达水平提供可靠工具。支持科研人员在肿瘤生物学、免疫学等领域开展实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    TNFRSF10C ELISA Kit; DCR1 ELISA Kit; LIT ELISA Kit; TRAILR3 ELISA Kit; TRID ELISA Kit; UNQ321/PRO366 ELISA Kit; Tumor necrosis factor receptor superfamily member 10C ELISA Kit; Antagonist decoy receptor for TRAIL/Apo-2L ELISA Kit; Decoy TRAIL receptor without death domain ELISA Kit; Decoy receptor 1 ELISA Kit; DcR1 ELISA Kit; Lymphocyte inhibitor of TRAIL ELISA Kit; TNF-related apoptosis-inducing ligand receptor 3 ELISA Kit; TRAIL receptor 3 ELISA Kit; TRAIL-R3 ELISA Kit; TRAIL receptor without an intracellular domain ELISA Kit; CD antigen CD263 ELISA Kit
  • 缩写：
    TRAIL-R3
  • Uniprot No.：
    O14798
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)625.668628.643626.411627.471624.154623.666SD0.0590.0480.0520.0610.0550.066CV(%)5.0044.0574.4075.1634.6735.611







  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL R3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)

    1:1

    Average %

    101

    Range %

    88-105

    1:2

    Average %

    104

    Range %

    95-109

    1:4

    Average %

    94

    Range %

    87-98

    1:8

    Average %

    104

    Range %

    99-108








  • 回收率：

    The recovery of human TRAIL R3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    106

    100-109

    EDTA plasma (n=4)

    101

    95-105








  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
































































    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.652

    2.504

    2.578

    2.408

    2500

    2.326

    2.242

    2.284

    2.114

    1250

    1.736

    1.765

    1.751

    1.581

    625

    1.170

    1.189

    1.180

    1.010

    312

    0.716

    0.698

    0.707

    0.537

    156

    0.515

    0.500

    0.508

    0.338

    78

    0.280

    0.281

    0.281

    0.111

    0

    0.168

    0.172

    0.170










  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×