首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人促甲状腺素(TSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TSH含量。TSH即促甲状腺激素，是腺垂体分泌的促进甲状腺生长和机能的激素。其在调节甲状腺功能中起关键作用。研究围绕其与甲状腺激素的反馈调节机制展开，探索其相关信号通路，以深入了解甲状腺疾病发病机制及为治疗提供新思路。试剂盒检测范围为0.3 uIU/mL-12 uIU/mL，灵敏度为0.15 μIU/mL。该产品适用于甲状腺功能相关动物模型研究、激素替代疗法药效评估、环境内分泌干扰物筛查等科研领域，也可用于体外培养细胞或组织样本中TSH分泌水平的动态监测，为代谢调控、生殖健康等基础医学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TSHBThyrotropin subunit beta ELISA kit; Thyroid-stimulating hormone subunit beta ELISA kit; TSH-B ELISA kit; TSH-beta ELISA kit; Thyrotropin beta chain ELISA kit; Thyrotropin alfa ELISA kit
缩写：TSH
Uniprot No.：P01222
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.3 uIU/mL-12 uIU/mL
灵敏度：0.15 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of human TSHspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average 122.2192.3542.287 61.2151.2541.235 30.6480.6640.656 1.50.3610.3540.358 0.60.1550.1580.157 0.30.0780.0790.079 | ||||||
货期：3-5 working days
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