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Human Tenascin-c (TNC) ELISA Kit

  • 中文名: 人类肌腱蛋白- c(TNC) ELISA Kit
货号: IPD-E13125h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人类肌腱蛋白- c(TNC)酶联免疫试剂盒检测样本中的TNC含量。本试剂盒可为研究其在伤口修复、癌症转移或免疫微环境中的作用提供可靠工具,适用于细胞生物学实验、病理模型分析或生物标志物探索等科研领域。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    150 225 ELISA Kit; Cytotactin ELISA Kit; Glioma associated extracellular matrix antigen ELISA Kit; Glioma-associated-extracellular matrix antigen ELISA Kit; GMEM ELISA Kit; GP 150 225 ELISA Kit; GP 150-225 ELISA Kit; GP ELISA Kit; Hexabrachion ELISA Kit; HXB ELISA Kit; JI ELISA Kit; MGC167029 ELISA Kit; Miotendinous antigen ELISA Kit; Myotendinous antigen ELISA Kit; Neuronectin ELISA Kit; TENA_HUMAN ELISA Kit; Tenascin ELISA Kit; Tenascin-C ELISA Kit; Tenascin-C isoform 14/AD1/16 ELISA Kit; TenascinC ELISA Kit; TN ELISA Kit; TN C ELISA Kit; TN-C ELISA Kit; TNC ELISA Kit
  • 缩写:
    TNC
  • Uniprot No.:
    P24821
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
  • 检测范围:
  • 灵敏度:
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



     Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)7.0567.3867.5797.0287.4777.432SD0.0400.0370.0420.0540.0480.047CV(%)5.0894.6545.4337.1246.4606.112







  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human TNC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    88-95


    1:2

    Average %

    102


    Range %

    97-105


    1:4

    Average %

    92


    Range %

    87-95


    1:8

    Average %

    99


    Range %

    95-102









  • 回收率:

    The recovery of human TNC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%


    Serum (n=5)

    94

    91-96


    EDTA plasma (n=4)

    94

    91-96









  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected


    200

    2.788

    2.816

    2.802

    2.677


    66.6

    2.166

    2.198

    2.182

    2.057


    22.2

    1.388

    1.422

    1.405

    1.280


    7.4

    0.738

    0.722

    0.730

    0.605


    2.46

    0.370

    0.354

    0.362

    0.237


    0.82

    0.265

    0.248

    0.257

    0.132


    0.27

    0.201

    0.192

    0.197

    0.072


    0

    0.126

    0.124

    0.125











  • 货期:
    3-5 working days

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