Rat Osteoprotegerin,OPG ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07404r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠骨保护素(OPG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF11B含量。TNFRSF11B是重要的骨代谢调节靶点。其背景在于参与破骨细胞分化、增殖等过程，对维持骨稳态至关重要。研究机制主要围绕其与配体相互作用，通过下游信号通路调控骨骼发育和重建，在骨质疏松等骨相关疾病研究中受关注。试剂盒检测范围为0.78 ng/ml- 50 ng/ml，灵敏度为0.195 ng/ml。适用于大鼠模型中的骨代谢机制研究、骨质疏松药物干预实验或骨组织病理学分析等科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tnfrsf11b ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoprotegerin ELISA Kit
缩写：
TNFRSF11B
Uniprot No.：
O08727
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.78 ng/ml-50 ng/ml
灵敏度：
0.195 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	98
	Range %	92-104
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
50	1.906	2.034	1.970	1.796
25	1.113	1.067	1.090	0.916
12.5	0.594	0.628	0.611	0.437
6.25	0.395	0.409	0.402	0.228
3.12	0.270	0.281	0.276	0.102
1.56	0.235	0.250	0.242	0.069
0.78	0.210	0.214	0.212	0.038
0	0.172	0.175	0.174