Human Osteoprotegerin,OPG ELISA KIT

中文名： 人骨保护素(OPG)ELISA KIT

货号: IPD-E04692h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人骨保护素(OPG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF11B含量。TNFRSF11B，即肿瘤坏死因子受体超家族11B，是一种在骨代谢中起调节作用的分泌型糖蛋白。其研究机制主要涉及与RANKL结合以抑制破骨细胞生成，参与骨重塑和癌症的多个生物过程。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。适用于科研领域对骨代谢调控机制、骨骼相关疾病模型建立、药物干预效果评估等研究，特别在骨质疏松动物模型构建、成骨细胞分化实验、炎症性骨破坏机制探讨等场景中，可为研究人员提供可靠的定量分析工具。试剂盒包含预包被抗体、检测抗体及全套实验缓冲体系，支持直接开展OPG蛋白表达水平的动态监测及多组学关联分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
MGC29565 ELISA Kit; OCIF ELISA Kit; OPG ELISA Kit; Osteoclastogenesis inhibitory factor ELISA Kit; Osteoprotegerin ELISA Kit; PDB5 ELISA Kit; TNF receptor superfamily member 11b ELISA Kit; TNFRSF 11B ELISA Kit; TNFRSF11B ELISA Kit; TR 1 ELISA Kit; TR1 ELISA Kit; TR11B_HUMAN ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit
缩写：
TNFRSF11B
Uniprot No.：
O00300
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
7.8 pg/mL-500 pg/mL
灵敏度：
1.95 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)62.56562.30062.79562.47762.30062.034SD0.0450.0400.0390.0560.0540.057CV(%)5.1314.5774.4346.3936.1786.544

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

91-105

1:2

Average %

Range %

80-92

1:4

Average %

102

Range %

97-108

1:8

Average %

Range %

86-98

回收率：

The recovery of human OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-99
EDTA plasma (n=4)	95	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













pg/ml	OD1	OD2	Average	Corrected
500	2.725	2.656	2.691	2.584
250	2.215	2.108	2.162	2.055
125	1.513	1.482	1.498	1.391
62.5	0.845	0.834	0.840	0.733
31.2	0.516	0.512	0.514	0.407
15.6	0.324	0.321	0.323	0.216
7.8	0.242	0.228	0.235	0.128
0	0.111	0.103	0.107