首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨保护素(OPG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF11B含量。TNFRSF11B是重要靶点。它参与调控骨代谢平衡，在破骨细胞生成和骨吸收过程中发挥关键作用。研究发现其异常与骨质疏松等骨疾病密切相关。目前对它的研究机制主要聚焦于其信号通路，有望为相关疾病治疗提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。广泛应用于骨代谢疾病模型构建、药物干预效果评估、骨骼发育研究及炎症相关信号通路探索等科研场景，其稳定性和特异性为体外实验提供可靠数据支持，是研究骨稳态调控机制及病理过程的理想工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfrsf11b ELISA Kit; Ocif ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoclastogenesis inhibitory factor ELISA Kit; Osteoprotegerin ELISA Kit
缩写：TNFRSF11B
Uniprot No.：O08712
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:10 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:20 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:40 | Average % | 97 | |||||
| Range % | 93-100 | ||||||
回收率：
| The recovery of mouse OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 96 | 92-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.0452.1022.0741.938 5001.3571.3081.3331.197 2500.8790.9150.8970.761 1250.5930.5780.5860.450 62.50.3810.3680.3750.239 31.20.2750.2980.2870.151 15.60.1930.1870.1900.054 00.1350.1370.136 | |||||||
货期：3-5 working days
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