Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

产品描述：猪肿瘤坏死因子alpha(TNF/TNFA/TNFSF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TNF含量。TNF（肿瘤坏死因子）是一种重要的细胞因子。背景上，它在免疫调节、炎症反应等生理过程中起关键作用。研究机制方面，TNF可与相应受体结合，激活多条信号通路，从而调控细胞的存活、增殖、凋亡等过程，其异常表达与多种疾病相关。试剂盒检测范围为0.047 ng/mL-3 ng/mL，灵敏度为0.040 ng/mL。该产品适用于科研领域中对猪免疫系统功能的探索，如评估病原体（如猪繁殖与呼吸综合征病毒、猪圆环病毒等）感染模型中细胞因子风暴的动态变化，或研究抗炎药物、免疫调节剂对TNFα表达的影响，为畜牧兽医研究、动物疾病模型构建及疫苗研发提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

别名：TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

缩写：TNF

Uniprot No.：P23563

种属：Sus scrofa (Pig)

样本类型：serum, plasma, cell culture supernates, tissue homogenates

检测范围：0.047 ng/mL-3 ng/mL

灵敏度：0.040 ng/mL

反应时间：1-5h

样本体积：50-100ul

检测波长：450 nm

研究领域：Immunology

测定原理：quantitative

测定方法：Sandwich

精密度：

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度：

 To assess the linearity of the assay, samples were spiked with high concentrations of pig TNFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

货期：3-5 working days