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产品描述:人转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的TGFB1含量。TGFB1(转化生长因子β1)是一种多功能细胞因子,最初被认为是诱导啮齿动物成纤维细胞生长的生长因子。它能够调节细胞增殖、生长、分化和细胞运动,同时具有免疫调节和促纤维化作用。在糖尿病肾病中,TGFB1通过激活SMAD信号通路,促进肾小球系膜细胞增殖和肾脏小管间质纤维化,是糖尿病肾病发病的关键机制之一。试剂盒检测范围为0.78 ng/mL-50 ng/mL,灵敏度为0.747 ng/mL。适用于体外培养细胞分泌功能研究、疾病模型动物血清指标分析等科研场景。科研人员可通过探究TGF - β1在肿瘤微环境调控、组织修复机制中的作用,或用于评估药物干预对TGF - β1信号通路的影响本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Cartilage-inducing factor ELISA Kit; CED ELISA Kit; Differentiation inhibiting factor ELISA Kit; DPD1 ELISA Kit; LAP ELISA Kit; Latency-associated peptide ELISA Kit; Prepro transforming growth factor beta 1 ELISA Kit; TGF beta 1 ELISA Kit; TGF beta ELISA Kit; TGF beta 1 protein ELISA Kit; TGF-beta 1 protein ELISA Kit; TGF-beta-1 ELISA Kit; TGF-beta-5 ELISA Kit; TGF-beta1 ELISA Kit; TGFB ELISA Kit; Tgfb-1 ELISA Kit; tgfb1 ELISA Kit; TGFB1_HUMAN ELISA Kit; TGFbeta ELISA Kit; TGFbeta1 ELISA Kit; Transforming Growth Factor b1 ELISA Kit; Transforming Growth Factor beta 1 ELISA Kit; Transforming growth factor beta 1a ELISA Kit; transforming growth factor beta-1 ELISA Kit; transforming growth factor, beta 1 ELISA Kit
缩写:TGFB1
Uniprot No.:P01137
种属:Homo sapiens (Human)
样本类型:serum, plasma, cell culture supernates
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.747 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
货期:3-5 working days
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