首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组织因子途径抑制物(TFPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TFPI含量。TFPI 即组织因子途径抑制物，是一种天然存在的抗凝蛋白。它能抑制组织因子介导的凝血途径的活化，在维持体内凝血平衡中起重要作用。相关研究围绕其抑制机制展开，探索其在血栓性疾病防治等方面的应用潜力。试剂盒检测范围为0.782 ng/mL-50 ng/mL，灵敏度为0.293 ng/mL。适用于体外培养细胞模型、动物实验样本或临床前研究中的TFPI水平检测；可为凝血机制研究、抗血栓药物开发、炎症相关血管病变或肿瘤转移机制等科研方向提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Anti convertin ELISA Kit; EPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; LACI ELISA Kit; Lipoprotein associated coagulation inhibitor ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; TFI ELISA Kit; TFPI 1 ELISA Kit; TFPI ELISA Kit; TFPI1 ELISA Kit; TFPI1_HUMAN ELISA Kit; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) ELISA Kit; Tissue factor pathway inhibitor ELISA Kit
缩写：TFPI
Uniprot No.：P10646
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.782 ng/mL-50 ng/mL
灵敏度：0.293 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 86 | 81-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.5192.6232.5712.440 252.0212.1542.0881.957 12.51.4341.4561.4451.314 6.250.8130.8380.8260.695 3.1250.4360.4450.4410.310 1.5630.3040.3100.3070.176 0.7820.2210.2160.2190.088 00.1300.1310.131 | ||||||
货期：3-5 working days
×
