首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：人转铁蛋白(TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织培养上清液、脑脊液（CSF）样本中的TF含量。试剂盒检测范围为0.023 nmol/L-1.5 nmol/L，灵敏度为0.006 nmol/L。本试剂盒可为铁代谢研究、疾病相关生物标志物筛选、药物开发中的细胞培养监测以及神经系统疾病的体液分析等科研领域提供可靠数据支持，尤其适用于跨样本类型的对比实验设计。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apotransferrin ELISA Kit; Beta 1 metal binding globulin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; DKFZp781D0156 ELISA Kit; PRO1400 ELISA Kit; PRO1557 ELISA Kit; PRO2086 ELISA Kit; Serotransferrin ELISA Kit; Serotransferrin precursor ELISA Kit; Siderophilin ELISA Kit; TF ELISA Kit; TFQTL1 ELISA Kit; Transferin ELISA Kit; Transferrin ELISA Kit; TRFE_HUMAN ELISA Kit
缩写：TF
Uniprot No.：P02787
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, cell culture supernates, cerebrospinal fluid (CSF)
检测范围：0.023 nmol/L-1.5 nmol/L
灵敏度：0.006 nmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:50000 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:100000 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:200000 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:400000 | Average % | 90 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of human TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 98 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/LOD1OD2AverageCorrected 1.52.3672.3962.3822.278 0.751.8981.7531.8261.722 0.3751.2621.2881.2751.171 0.1870.9480.9820.9650.861 0.0940.5830.5970.5900.486 0.0470.3530.3480.3510.247 0.0230.2840.2950.2900.186 00.1020.1060.104 | ||||||
货期：3-5 working days
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