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Human Trefoil factor 3(TFF3) ELISA kit

  • 中文名： 人三叶因子3(TFF3)ELISA kit
货号: IPD-EL023433HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人三叶因子3(TFF3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的TFF3含量。TFF3 即三叶因子 3，是三叶因子家族成员，在胃肠道等组织表达，参与黏膜修复与保护。研究机制上，其能与特定受体相互作用，激活信号通路，调节细胞增殖、迁移和黏附等，还与肿瘤发生发展、炎症性疾病进程相关，是潜在治疗靶点。试剂盒检测范围为0.312 ng/ml-20 ng/ml.，灵敏度为0.078 ng/ml.。消化道疾病机制研究、肿瘤微环境分析、炎症模型评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    hITF ELISA Kit; hP1.B ELISA Kit; Intestinal trefoil factor ELISA Kit; ITF ELISA Kit; mITF ELISA Kit; OTTMUSP00000021729 ELISA Kit; P1B ELISA Kit; Polypeptide P1.B ELISA Kit; TFF3 ELISA Kit; TFF3_HUMAN ELISA Kit; TFI ELISA Kit; TREFOIL ELISA Kit; Trefoil factor (intestinal) ELISA Kit; Trefoil factor 3 ELISA Kit
  • 缩写：
    TFF3
  • Uniprot No.：
    Q07654
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.312 ng/ml-20 ng/ml.
  • 灵敏度：
    0.078 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TFF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    84-96


    1:2

    Average %

    93


    Range %

    89-97


    1:4

    Average %

    97


    Range %

    94-101


    1:8

    Average %

    92


    Range %

    91-101


  • 回收率：

    The recovery of human TFF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-100


    EDTA plasma (n=4)

    96

    87-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    1.821

    1.857

    1.839

    1.679


    10

    1.319

    1.281

    1.300

    1.140


    5

    0.794

    0.787

    0.791

    0.631


    2.5

    0.488

    0.517

    0.503

    0.343


    1.25

    0.364

    0.342

    0.353

    0.193


    0.625

    0.250

    0.259

    0.255

    0.095


    0.312

    0.206

    0.203

    0.205

    0.045


    0

    0.159

    0.161

    0.160




  • 货期：
    3-5 working days

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